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Immune modulatory effects of IL-22 on allergen-induced pulmonary inflammation

Fang, P and Zhou, L and Zhou, Y and Kolls, JK and Zheng, T and Zhu, Z (2014) Immune modulatory effects of IL-22 on allergen-induced pulmonary inflammation. PLoS ONE, 9 (9).

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Abstract

IL-22 is a Th17/Th22 cytokine that is increased in asthma. However, recent animal studies showed controversial findings in the effects of IL-22 in allergic asthma. To determine the role of IL-22 in ovalbumin-induced allergic inflammation we generated inducible lung-specific IL-22 transgenic mice. Transgenic IL-22 expression and signaling activity in the lung were determined. Ovalbumin (OVA)-induced pulmonary inflammation, immune responses, and airway hyperresponsiveness (AHR) were examined and compared between IL-22 transgenic mice and wild type controls. Following doxycycline (Dox) induction, IL-22 protein was readily detected in the large (CC10 promoter) and small (SPC promoter) airway epithelial cells. IL-22 signaling was evidenced by phosphorylated STAT3. After OVA sensitization and challenge, compared to wild type littermates, IL-22 transgenic mice showed decreased eosinophils in the bronchoalveolar lavage (BAL), and in lung tissue, decreased mucus metaplasia in the airways, and reduced AHR. Among the cytokines and chemokines examined, IL-13 levels were reduced in the BAL fluid as well as in lymphocytes from local draining lymph nodes of IL-22 transgenic mice. No effect was seen on the levels of serum total or OVA-specific IgE or IgG. These findings indicate that IL-22 has immune modulatory effects on pulmonary inflammatory responses in allergen-induced asthma.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Fang, P
Zhou, L
Zhou, Y
Kolls, JKjkk23@pitt.eduJKK23
Zheng, T
Zhu, Z
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorRyffel, BernhardUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 25 September 2014
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 9
Number: 9
DOI or Unique Handle: 10.1371/journal.pone.0107454
Schools and Programs: School of Medicine > Pediatrics
Refereed: Yes
Other ID: NLM PMC4177833
PubMed Central ID: PMC4177833
PubMed ID: 25254361
Date Deposited: 08 May 2015 15:32
Last Modified: 02 Apr 2021 21:55
URI: http://d-scholarship.pitt.edu/id/eprint/24021

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